The ecoregionalization based on lacustrine nutrients is the basis of nutrient benchmark and trophication control criteria of lakes, and is also the scientific basis and magnitude pathway of compositive evaluation, prevention, control and management. In this paper, after analyzing the concepts on ecoregion, hydroecoregion, and discussion on the base of classification, the lacustrine response to nutrients in geographic sections, driving factors and models, we proposed a Chinese hydroecoregional frame based on regionalization, classification and phase. By analyzing the environmental factors that influencing trophic status, we determined the criterions for lake regionalization, including by climate zone, topography and water system; by topography, soil nutrients, land use, shape of lake basin (water depth, surface area, development of lake shore) and hydrologic characteristics (residence time, water level, degree of mineralization); by nutrient content (nitrogen, phosphorus et al), total primary productivity( biomass of phytoplankton, attached alga and aquatic macrophyte), and by dominant plant types (phytoplankton and macrophyte). We primarily constructed indices system of ecoregionalization for Chinese lakes and this work lays a basis of lake ecoregionalization based on nutrients in China.