Using fluorescent dye acridine orange (AO) as a fluorescent probe and CdTe as an internal standard, we assembled AO and CdTe quantum dots on the surface of silanized quartz respectively. The assembled condition have been discussed. The optimal concentration of CdTe is 8. 33 mmol · L-1 , CS is 1.0 g · L-1 and AO is 10 μnol · L-1. These SAMs can well avoid the disturbance from the environmental factors, such as the power of the equipment, slit ratio, and improve the accuracy of fluorescence analysis.
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