Valproic Acid Promotes Differentiation of Mouse Neural Stem Cells Into Neurons and Its Mechanism of Effect
To investigate the possible mechanisms by which valproic acid(VPA)promotes the differentiation of mouse neural stem cells(NSCs)into neurons,neural stem cell were isolated from mouse brain and passaged in culture,then re-inoculated in different types of differentiation medium to induce cell differentiation into neu-rons,and the expression of neuron-specific markers was detected by immunofluorescence staining,and the ex-pression levels of key molecules in the Wnt/β-catenin signalling pathway were investigated before and after VPA treatment by qRT-PCR.The results showed that the proportion of Nestin and Pax6 positive cells within suspensions of NSCs cultured in passages after isolation was high,the differentiated cells showed significant neuronal morphology,and the expression levels of neuronal markers β-Tubulin(TUJ1),Doublecortin(DCX)and recombinant microtubule associated protein 2(MAP2)were significantly increased after differentiation.Compared with the normal group,the mRNA relative expression levels of Wnt-3α,β-catenin and cyclin D1 was increased,and the mRNA relative expression levels of p21 protein was decreased in the VPA-treated group.FH535,an inhibitor of the Wnt/β-catenin signalling pathway,significantly attenuated the differentia-tion of NSCs into neurons in VP A.
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