Effects of Hyperoxia on Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel in High Oxygen-induced Injury of Alveolar Epithelial Cells Type Ⅱ
Objective To investigate the role of cystic fibrosis transmembrane conductance regulator(CFTR)chloride channel in high oxygen-induced injury of alveolar epithelial cells type Ⅱ(AECⅡ).Mfethods AECsⅡ were Placed in a sealed chamber containing 95％O2 and 5％CO2,and incubated in a culture incubator for 24 hours or 48 hours to establish a model of high oxygen damage in AECsⅡ.In the experimental group,AECsⅡ were randomly divided into the following groups:the hyperoxia group for 24 hours,the hyperoxia group for 24 hours plus the stimulant(Gen),the hyperoxia group for 24 hours plus the inhibitor(AA),the hyperoxia group for 48 hours,the hyperoxia group for 48 hours plus Gen,and the hyperoxia groupfor 48 hours plus AA.The control group AECsⅡ were cultured in a medium with air and without intervention.The whole-cell Patch-clamp system was used to record the chloride current strength of CFTR which was recorded after Gen at 50μM or AA at 10μM was added in the extracellular fluid.Data was analyzed using Clampfit 10.6,where the current changes under different stimulation voltages were calculated,and the results were presented in the form of a voltage-current trend line.Spearman correlation analysis was conducted on the different groups using R 4.4.1 statistical software.Results 1.ComPared with the control group,high oxygen exposure for 24 hours and 48 hours significantly inhibited the chloride current of AECⅡ,showing a decrease in the negative value(P＜0.01),with the 24-hour group showing a more significant inhibition.2.Compared with the simple hyPeroxia group,Gen significantly increased the chloride current of AECⅡ in high oxygen for 24 hours and 48 hours,showing an increase in the negative value(P＜0.01),with the 48-hour group being more obvious(P＜0.01);while AA significantly inhibited the chloride current,showing a decrease in the negative value(P＜0.01),with the 48-hour group being more obvious(P＜0.01).Conclusion Hyperoxia may exert its effects by affecting the chloride current outflow of CFTR in AECⅡ.
Alveolar Epithelial Cells tyPe ⅡHyperoxiaCystic Fibrosis Transmembrane Conductance RegulatorGenisteinArachidonic Acid
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