Objective To investigate the relationship between miR-106a,miR-92a,miR-31 and neovascularization,invasion and metastasis in colorectal cancer(CRC).Methods 100 patients with colorectal cancer diagnosed and treated in our hospital from January 2018 to January 2020 were selected as CRC group,and another 60 healthy patients during the same period were selected as control group.Real-time fluorescence quantitative polymerase chain reaction(RT-PCR)was used to detect the expression levels of miR-106a,miR-92a and miR-31,and immunohistochemical method was used to detect the expression of CD34,and microvascular density(MVD)was calculated.The data of tumor invasion and metastasis were collected after 3 years of follow-up.The relationship between miR-106a,miR-92a and miR-31 and CRC neovascularization,invasion and metastasis was analyzed.Results The expression levels and MVD of miR-106a,miR-92a and miR-31 in CRC group were higher than those in control group(P＜0.05).Pearson correlation analysis showed that the expression levels of miR-106a,miR-92a and miR-31 were positively correlated with MVD in CRC patients(P＜0.05).Univariate analysis showed that the number of patients with high and moderate differentiation and the number of patients with TNM stage Ⅲ and Ⅳ in the invasive metastasis group was higher than that in the non-invasive metastasis group,and the preoperative expression levels of miR-106a,miR-92a and miR-31 were higher than those in the non-invasive metastasis group(P＜0.05).Multiple stepwise regression analysis showed that the degree of tumor differentiation,TNM stage,preoperative miR-106a expression level,preoperative miR-92a expression level,and preoperative miR-31 expression level were all independent influencing factors of tumor invasion and metastasis in CRC patients(P＜0.05).Conclusion miR-106a,miR-92a and miR-31 are highly expressed in colorectal cancer tissues,and are closely related to angiogenesis,invasion and metastasis of colorectal cancer.
Colorectal CancerInvasion and MetastasisAngiogenesismiR-106amiR-92amiR-31
维普
万方数据
