Objective To investigate the expression of pairing-related homeobox 1(PRRX1)protein in colorectal adenocarcinoma tissue and its relationship with microsatellite instability(MSI).Methods The clinical data of 168 patients with colorectal adenocarcinoma admitted to our hospital from October 2020 to December 2022 were retrospectively analyzed.Colorectal adenocarcinoma tissues and normal tissue adjacent to the cancer(≥5cm away from cancer)were collected during operation.The expression of PRRX1 protein in colorectal adenocarcinoma tissues and corresponding normal tissue adjacent to the cancer was detected by immunohistochemistry,and the relationship between PRRX1 protein expression and clinicopathological features was analyzed.The MSI of colorectal adenocarcinoma tissues was detected by PCR and divided into three groups of microsatellite high instability(MSI-H),microsatellite low instability(MSI-L)and microsatellite stability(MSS).The expression of PRRX1 protein among the three groups was compared.Spearman was used to analyze the relationship between PRRX1 protein expression and MSI in patients with colorectal adenocarcinoma.Results The positive expression rate of PRRX1 protein in colorectal adenocarcinoma tissues was higher than that in normal tissue adjacent to the cancer(P＜0.05).The positive expression rates of PRRX1 protein in patients with low differentiation,clinical stage Ⅲ+Ⅳ and lymph node metastasis were higher than those in patients with medium and high differentiation,clinical stage Ⅰ+Ⅱ and no lymph node metastasis(P＜0.05).Among 168 patients with colorectal adenocarcinoma,120 cases(71.43％)were diagnosed as MSS,and 28 cases(16.67％)as MSI-H and 20 cases(11.90％)as MSI-L.The positive expression rate of PRRX1 in MSI-H group was higher than that in MSS group(P＜0.05).Spearman analysis showed that PRRX1 protein expression was positively correlated with MSI(r=0.568,P=0.023).Conclusion PRRX1 protein is highly expressed in colorectal adenocarcinoma tissues,which is related to the degree of tumor differentiation,clinical stage and lymph node metastasis,and it is closely related to the occurrence of MSI in patients.
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