青州银瓜及其近缘种的RAPD研究
A Study on Genetic Polymorphism of Qingzhou Silver Melon and its Related Species Using RAPD
郭晓丽 1杨晓婷 1刘思萌 1刘芯瑜1
作者信息
- 1. 衡水学院 生命科学学院,河北 衡水 053000
- 折叠
摘要
青州银瓜品相优良、甘甜多汁、香气浓郁,是全国名优瓜果蔬菜品种之一.采用 RAPD 标记技术对青州银瓜及其近缘种的9种供试材料进行基因组分析,并构建了 9种供试材料的聚类分析图和主坐标分析图.扩增结果显示,在选取的30个RAPD随机引物中,有24个随机引物共扩增出276条条带,其中多态性条带有176条,平均每个引物获得多态性条带7.3条,平均多态性比率为63.77%.聚类分析与主坐标分析结果显示,9 种供试材料的遗传相似系数分布在 0.61～0.98 之间,表明供试材料之间存在一定的遗传差异,其中M1(三叶酥花皮菜瓜)与其他供试材料之间的遗传相似系数较小,亲缘关系较远;M5(青州银瓜)、M4(白皮脆甜羊角蜜)、M6(火凤凰银瓜)的遗传相似系数较高,这 3 个种质的亲缘关系最为接近.
Abstract
Qingzhou silver melon has excellent quality,sweetness,juiciness,and strong aroma,making it one of the national famous and high-quality varieties of melons and vegetables.Using RAPD marker technology,genomic analysis was conducted on 9 test materials of Qingzhou silver melon and its closely related species,and clustering analysis and principal coordinate analysis graphs of the 9 test materials were constructed.The amplification results showed that among the selected 30 RAPD random primers,24 primers amplified a total of 276 bands,of which 176 bands were polymorphic,with an average of 7.3 polymorphic bands obtained per primer and an average polymorphism rate of 63.77%.The results of clustering analysis and principal coordinate analysis indicated that the genetic similarity coefficients of the 9 test materials ranged from 0.61 to 0.98,suggesting certain genetic differences among the test materials.Among them,M1(three-leaf crispy flower skin melon)had a smaller genetic similarity coefficient with other test materials,indicating a more distant relationship;while M5(Qingzhou silver melon),M4(white-skinned crispy sweet horn honey),and M6(fire phoenix silver melon)had higher genetic similarity coefficients,indicating that these three germplasm are the most closely related.
关键词
青州银瓜/近缘种/RAPD/聚类分析/主坐标分析Key words
Qingzhou silver melon/closely related species/RAPD/cluster analysis/principal coordinate analysis引用本文复制引用
出版年
2025
NETL
NSTL
万方数据