Establishment of the Tissue Culture and Fast Multiplication System for Red-leaf Photina
The strong new axillary buds of healthy red-leaf photinia grown in the nursery of Hengshui Jinshu Landscape Greening Technology Co,Ltd.were selected as explants,and explore the effects of different disinfection methods,different medium formulations,and different acclimatization and transplantation conditions on the tissue culture fast multiplication system of red-leaf photinia.The study aims to identify the optimal disinfectant,disinfection time,and adventitious bud differentiation medium formulation for red-leaf photinia explants,as well as the optimal rooting medium formulation and substrate combination for red-leaf photinia tissue culture seedlings,providing technical support for the large-scale cultivation of red-leaf photinia.The research results are as follows:0.1%HgCl2 treatment for 7 minutes has the best disinfection effect on red-leaf photinia explants,with a low pollution rate of 13.3%and the highest survival rate of 57.8%.The optimal medium for adventitious bud differentiation is MS+2.0mg/L 6-BA+0.2mg/L NAA+0.4mg/L KT,with an adventitious bud induction rate of up to 83%.The most suitable rooting medium is 1/2 MS+0.1mg/L NAA+0.5mg/L IBA,with a rooting rate of 96%and an average root length of 7.66cm.The best transplanting substrate combination is garden soil:leaf mold:perlite=5:4:1,with a high survival rate of seedlings up to 92%.A fast multiplication technology system for red-leaf photinia tissue culture seedlings has been established preliminarily,providing a theoretical basis for achieving large-scale production.
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