Study on the Improvement of HPLC Method for Analyzing Endogenous Hormone in Peanut
This experiment improved the method of extraction, purification and analysis with high-performance liquid chromatography ( HPLC) on endogenous hormones auxin (IAA), abscisic acid (ABA), gibberellic acid (GA) and 6-Benzylaminopurine (6-BA) content in peanut kernel.To deal with the existence of large amount of fat and phenolic, the test materials were grinded in liquid nitrogen and lixiviated in 80% methanol, then pigment was eliminated in chloroform, phytohormones were extracted in ethyl acetate, phenolic was compounded in polyving pyrrolidone (PVPP).Detection was made with UV detector at 254 nm.
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