茉莉酸和乙烯信号转导途径参与花生应答果腐病病原菌侵染的转录组测序分析
Transcriptomic Profiling Reveals the JA and ET Signal Transduction during Field Peanut Pod Rot Pathogen Infection
王冕 1王通 1陈明娜 1杨珍 1禹山林 1陈娜 1许静 1潘丽娟 1张朝昕 2郭鑫钢 3迟晓元1
作者信息
- 1. 山东省花生研究所,山东 青岛 266100
- 2. 青岛市市容环境卫生管理中心,山东 青岛 266071
- 3. 邹平市综合检验检测中心,山东 邹平 256200
- 折叠
摘要
花生果腐病是花生生产上的一种重要病害,对花生的产量和品质构成了严重威胁.本研究以花育20号感病前后籽仁为样品,进行转录组测序,得到Unigene 60756个,其中表达量上调的基因10147个,下调的基因4334个;GO富集分析表明,差异表达基因被分类到24种细胞组分、5种分子功能和23种生物过程中;KEGG富集分析发现有4291个差异基因被注释到34条代谢通路中.筛选得到多条花生响应果腐病相关的激素信号转导途径,通过分析相关基因的表达图谱,推测花生可能通过JA/ET介导的抗病途径来抵抗果腐病病原菌的侵染.
Abstract
Pod rot, an important disease affecting peanut production, causes serious damage to pea-nut yield and quality.In this study, RNA sequencing analysis was performed with peanut cultivar Huayu20 kernels that infected with pod rot disease in field, and that uninfected kernels acting as control. As a result, a total of 60,756 unigenes were obtained, among which the expression of 10,147 genes were up-regulated and 4,334 genes were down-regulated.Further, Gene Ontology enrichment analysis showed that the differentially expressed genes (DEGs) were assigned to 24 cellular components, 5 molecular func-tions and 23 biological processes.Through Kyoto Encyclopedia of Genes and Genomes enrichment analy-sis, a total of 4,291 DEGs were mapped to 34 metabolic pathways.We identified multiple hormone sig-nal transduction pathways that were responsive to pod rot disease infection.Based on the expression pattern of related genes, we speculate that peanuts may resist pod rot disease infection through jas-monic acid-and ethylene-mediated pathways.
关键词
花生/果腐病/转录组测序/JA/ET信号转导途径Key words
peanut/pod rot disease/transcriptome/JA and ET signal transduction pathway引用本文复制引用
基金项目
国家自然科学基金(31701464)
国家花生产业技术体系项目(CARS-13)
泰山学者工程专项(tsnq201812121)
山东省自然科学基金(ZR2020MC103)
山东省农业科学院农业科技创新工程(CXGC2016B02)
山东省农业科学院农业科技创新工程(CXGC2018E21)
山东省重大科技创新工程项目(2019JZZY010702)
广东省重点领域研发计划项目(2020B020219003)
青岛市科技惠民示范引导专项(20-3-4-26-nsh)
临沂市重点研发计划(2019YD009)
出版年
2021
国家科技期刊平台
NSTL
维普
万方数据