Effects of Fungicides and Rhizobia Agents Seed Dressings on the Rhizospheric Microorganisms and Yield of Peanut
In order to screen biocides that can replace chemical agents and promote the green pro-duction of peanut,Xianghua2008,the mainly cultivated variety in Hunan,was taken as the test mate-rial in this experiment,and the field experiment of seed dressings with chemical biocide(thiophanate-methyl)and biocides(Trichoderma harzia and Bacillus amylus)mixed alone and mixed with rhizo-bium agents was carried out.The quantity of peanut rhizospheric microorganisms were measured from seedling stage to pod-bearing stage,and the pod characters and yield were measured at harvest stage.The results showed:1)Seed dressing with thiophanate-methyl could significantly promote the growth of bacteria.Seed dressing with thiophanate-methyl single-mixing,rhizobium agent single-mixing and three kinds of compound-mixing showed better performance in inhibiting fungi and promoting rhizobi-um.The growth promotion effect of seed dressing with T.harziensis single-mixing and B.amyloides single-mixing on actinomycetes was more obvious.The growth and proliferation of Aspergillus flavus were significantly inhibited by B.amylolytica single-and compound-mixing seed dressings.2)From the perspective of microbial community structure,the seed dressing of thiophanate-methyl single-mix-ing could increase the ratios of bacteria/fungi and actinomycetes/fungi during the whole growth stages of peanut,and the seed dressings of T.harziensis single-mixing and B.amylolytica single-mixing could increase the ratio of actinomycetes/fungi during the seedling and pod-bearing stages.3)Except for seed dressing with T.harziensis single-and compound-mixing,all other seed dressing treatments could increase peanut yield,among which the seed dressing with B.amylolytica single-mixing in-creased the pod yield by 49.39%,and the yield increase was 40.24%with rhizobium single-mixing and 32.78%with B.amylolyticus compound-mixing seed dressing.The conclusion can be summarized as:the seed dressing with single-mixing of thiophanate-methyl,compound-mixing of T.harziensis and rhizobium,compound-mixing of B.amylolyticus and rhizobium have better comprehensive microbial performance,and are more suitable for improving the microbial community in rhizosphere soil.Seed dressing with single-and compound-mixing of B.amylolytica and rhizobium mixing can increase the pod yield greatly.Therefore,seed dressing with B.amylolyticus single-and compound-mixing,and rhizobium agent mixing are recommended for extension in production.