Biocontrol Effect of Bacillus amylolyticus B235 on Peanut Root Rot
Using functional microorganisms to control peanut root rot is an environmentally friendly,green and efficient prevention and control measure.In this study,pot experiment and field plot experiment were carried out to observe the incidence of peanut root rot disease and the control effect of Bacillus amylolyticus B235 on Fusarium.The results of pathogen inoculation showed that after inoculation with Fusarium,peanut seed germination was slow,the taproot and lateral roots were short,and the incidence was high,and the worst seed emergence rate was only 28.2％according to the mixed treatment of F1 and F2 strains.The results of pot experiment showed that,compared with the pathogen inoculation treatment,the germination rate and seedling emergence rate of peanut after inoculation with biocontrol bacteria B235 were significantly increased,and the germination rate and seedling emergence rate were both above 60％,with the highest result reached to 68.5％and 65.3％,re-spectively.The disease index of root rot decreased significantly,and the control effect was above 60％,with the highest result reached to 69.5％and 65.5％,respectively.The plot test results showed that compared with the control treatment,the peanut under the biocontrol treatment showed higher seedling emergence rate and well-developed root system;at the flowering stage,the incidence was 4.5％,which was signifi-cantly lower than 6.7％in conventional treatment and 10.5％in blank control;at the mature stage of peanut,the incidence of Fusarium root rot in the control treatment was serious(19.8％),while the incidence of Fusarium root rot in the biocontrol B235 treatment group was light(5.0％),significantly lower than that of the conventional treatment(7.3％),and the yield increase was significant;it was further found that the inoculation with strain B235 had positive effects on the population structure and diversity of rhizosphere microorganisms.These results indicated that the biocontrol bacteria B235 could significantly reduce the occurrence of root rot,promote the growth and increase the yield of pea-nut,and presented a strong prospect on its extension and application.
Bacillus amylolyticuspeanutroot rotFusariumbiological control
万方数据
维普
