Mapping of Candidate Testa Color Genes in Peanuts Based on BSA-Seq
The testa color of peanuts is an important agronomic trait,which is closely related to the content of flavonoids,such as anthocyanins.Cloning and application of genes related to testa color are crucial for breeding and developing new peanut varieties with improved nutritional properties.In this study,the peanut with purple testa color'T371'and the peanut with pink testa color'T34'were used as parents to construct an F2 segregating population.Based on high-throughput sequencing tech-nology,312.96 G of high-quality Clean Reads data were obtained in both parental and segregating pop-ulations.The BSA-seq approach was applied to identify a main SNPs and InDels regulatory correlation region with interval lengths of 7.61 Mb on chromosome 12,which contained 675 genes,among which 14 genes were found with non-synonymous mutation and 3 genes were detected with frame shift muta-tion.Based on gene annotation,arahy.AFLD8J,arahy.26781N,arahy.YQ76T0,arahy.1KM4NQ and arahy.X3FWT9 may be candidate genes for regulating testa color.This study is helpful for cloning genes related to peanut testa color and breeding new varieties.
万方数据
维普
