Identification and Analysis of Three Gene Families in Gibberellin Metabolic Pathway of Peanut
In order to explore the functions of DELLA,GIBBERELLIN INSENSITIVE DWARF(GID1)and GIBBERELLIN INSENSITIVE DWARF 2(GID2)gene families in peanut,bioinformatic strategies were used to analyze these genes in the metabolic pathway of gibberellin in terms of their physical and chemical properties,location information,gene structure,protein structure,conserved motif,phyloge-netic tree,cis-elements and gene expression mode.As the result,six DELLA genes in the corresponding family were identified,with common conserved motif,DELLA or DELLA superfamily domains,as well as a single exon contained.The GID1 gene family in peanut had 57 members,which contained Abhydrolase_3 do-mains and three common conserved motifs.Among the eleven GID2 genes in peanut,a single exon and F-box domains as well as three common conserved motifs were shared.Three gene families are unevenly distributed on chromosomes,and most of them are distributed on the ends of chromosomes.The phylogenetic analysis showed that the above three genes were closely related to their counterparts in soybean,and their promoter regions contained a large number of cis-elements,among which 51.35％of the relative gene members had gibberellin-responsive elements.Most of genes containing gibberellin-responsive elements were expressed at low levels in various tissues,and some genes were mainly expressed at pattee stage of the seed and pericarp.In conclusion,the bioinformatic analyses of DELLA,GID1 and GID2 gene families will lay a foundation for further study of the three gene families involved in the gibberellin metabolism pathway in peanut.
peanutDELLAGID1GID2gene familybioinformatics analysismetabolic pathway of gibberellin
万方数据
维普
