Cloning,Expression Analysis,and Interaction Protein Identification of AhHSP90-B8 Gene from Peanut
The Heat Shock Protein 90(HSP90)genes play important roles in plant growth,de-velopment,and disease resistance response.In this study,a HSP90 gene was cloned,its CDS is 2,100 base pairs(bp)in length,encoding 699 amino acids,with a protein molecular weight(MW)of 80.2 kDa and an isoelectric point(pI)of 4.42,and there was no transmembrane structure.Subcellular lo-calization prediction results showed that the protein was hydrophilic and mainly located in the endo-plasmic reticulum.Nineteen known cis-acting elements related to plant growth,development,and stress resistance were in the promoter region.qRT-PCR analysis revealed that the AhHSP90-B8 gene was expressed in different tissues of peanut,with a relatively higher expression level in roots and pegs.There were significant differences in the expression levels at different stages after late spot dis-ease pathogens infection.Except for the relatively minor change in gene expression at the third day(3 days)after infection,gene expression levels showed significant up-regulation at other stages(5,10,15,and 20 days),reaching a peak after 15 days after infection.Furthermore,SGT1 was identified as an in-teracting protein,and was further verified their true interaction relationship through yeast two-hybrid.These results suggested that AhHSP90-B8 may act in conjunction with SGT1 by forming a protein complex.This study lays a theoretical foundation for further elucidating the function of the AhHSP90-B8 gene and provided potential resistance gene resources for breeding excellent new peanut varieties.
peanutAhHSP90-B8late leaf spot diseasemolecular characteristicsexpression analysisinteraction protein
万方数据
维普
