模拟微重力环境对皮肤干细胞增殖影响的研究
Research on the effects of simulated microgravity on the proliferation of skin stem cells
王思远 1赵映松 1谭映军 2施鏐佳 2殷学民 2陈江海1
作者信息
- 1. 华中科技大学同济医学院附属协和医院,武汉 430014
- 2. 中国航天员科研训练中心航天医学基础与应用国家重点实验室,北京 100080
- 折叠
摘要
目的 研究皮肤干细胞在模拟微重力(SMG)环境下的增殖能力与干性变化.方法 以SD大鼠皮肤干细胞为模型,建立悬浮培养体系,对比SMG环境与正常重力(1G)环境下皮肤干细胞的增殖及干细胞标志物的表达差异.结果 SMG环境显著影响悬浮培养环境下的皮肤干细胞球增殖速度,其增殖率较1G重力组约高12%.转录组测序结果显示SMG组1 673个基因上调,1 409个基因下调;Calcium signaling、cytokine-cytokine receptor interaction及PPAR pathway等重要信号通路在两个环境下表达差异显著.结论 SMG环境可通过调节关键信号通路的表达,影响悬浮培养环境下皮肤干细胞的增殖行为,为SMG环境下进一步研究工作提供了实验依据.
Abstract
Objective To study the changes and mechanism of skin stem cells in microgravity.Methods The skin stem cells of SD rats were used to establish a suspension culture system and compare the proliferation of skin stem cells with 1G gravity.Results The simulated microgravity significantly affected the velocity of skin stem cell sphere proliferation in suspension culture,which was about 12%higher than the 1G gravity group.Transcriptome sequencing showed that 1673 genes were up-regulated and 1409 genes were downregulated;Calcium signaling;cytokine-cytokine receptor interaction and PPAR pathway were different in the two environments.Conclusion Simulation of microgravity can affect the proliferation behavior of skin stem cells in suspension culture by regulating the expression of key signaling pathways,which provides an experimental basis for further research in spatial microgravity environ ment.
关键词
模拟微重力/皮肤干细胞/增殖/悬浮培养Key words
simulated microgravity/skin stem cells/proliferation/suspension culture condition引用本文复制引用
出版年
2024
NETL
NSTL
万方数据