微重力对休眠成肌细胞的影响
The effect of microgravity on hibernating myoblasts
刘一洲 1曹晓健 1施镠佳 2陈云强 1谭映军 2黄丹霞 1王春艳 2周秋至 3曲丽娜 2罗红梅 1殷学民 2张松 1刘朝霞 2李雅洁 3徐佳 3李莹辉 2陈红3
作者信息
- 1. 华中科技大学同济医学院附属同济医院康复医学科,武汉 430000
- 2. 中国航天员科研训练中心航天医学全国重点实验室,北京 100094
- 3. 华中科技大学同济医学院附属同济医院康复医学科,武汉 430000;华中科技大学大学同济医学院附属同济医院干细胞研究中心,武汉 430000
- 折叠
摘要
目的 研究微重力环境对休眠成肌细胞的影响.方法 利用真实微重力及模拟微重力培养成肌细胞10 d后,通过RNA-seq检测及免疫荧光研究微重力环境对成肌细胞基因表达及细胞生长的影响.结果 休眠成肌细胞在微重力环境中与增殖相关基因表达上调,相对于正常重力组,模拟微重力组细胞数量更多且Ki67阳性率更高.结论 微重力可以促进休眠成肌细胞的增殖.
Abstract
Objective To investigate the effects of microgravity environment on hibernating myoblasts.Methods Hibernating myoblasts were cultured under real and simulated microgravity conditions for 10 days.RNA-seq analysis and immunofluorescence are used to analysis the impact of microgravity environment on cell growth and gene expression of myoblasts.Results Under the microgravity conditions,genes associated with proliferation were upregulated.Under simulated microgravity,there were more and higher proportion of Ki67 positive cells compared to normal gravity conditions.Conclusion The microgravity environment promotes the proliferation of hibernating myoblasts.
关键词
休眠状态/成肌细胞/微重力/太空飞行/骨骼肌再生Key words
hibernation/myoblasts/microgravity/space flight/skeleton muscle regeneration引用本文复制引用
出版年
2024
NETL
NSTL
万方数据