To explore the application effect of PBL mixed teaching mode in medical nursing teaching.a total of 104 nursing students were selected as study subjects and divided into control group and obser-vation group.The observation group adopted the problem-based teaching method(PBL),combined with the online and offline teaching mode,and the control group adopted the traditional teaching mode.The fi-nal scores and students'self-evaluation learning effects of the two groups were compared.It was found that the students in the observation group had significantly higher final grades and recognition of learn-ing effect than those in the control group(P<0.05).PBL mixed teaching mode is helpful to improve the teaching quality and learning effect of medical nursing.