适配体AS1411修饰的管状DNA瓦在卵巢癌靶向治疗中的应用
Application of aptamer AS1411-modified tubular DNA tiles in targeted therapy of ovarian cancer
阮珞珊 1孙格格 1韩丽婷 1李新1
作者信息
- 1. 武汉大学人民医院妇Ⅱ科 湖北 武汉 430060
- 折叠
摘要
目的:研究AS1411适配体修饰的DNA瓦HA-6AS对卵巢癌细胞的靶向性以及载阿霉素(DOX)后对卵巢癌细胞的靶向治疗作用.方法:先用FAM荧光标记AS1411适配体或AS-like,与卵巢癌A2780细胞孵育3 h后离心去除游离的HA-6(FAM-AS)或H A-6(FAM-AS-like),用流式细胞术测定细胞的荧光强度;HA-6AS与DOX孵育过夜并高速离心去除游离的DOX,A2780细胞种于96孔板后培养过夜,将系列浓度的HA-6AS-DOX、AS1411+DOX和游离DOX加入96孔板,3 h后加入CCK-8试剂孵育,于酶标仪下测定吸光度并计算细胞的活力值.结果:用HA-6(FAM-AS)孵育的A2780细胞平均荧光强度比HA-6(FAM-AS-like)高(P＜0.000 1),且具有浓度依赖性;HA-6AS-DOX能在3 h内比AS1411+DOX和游离DOX更快速地对A2780细胞产生杀伤作用(P＜0.01).结论:AS1411能提高HA的靶向性,使HA-6AS-DOX可以对卵巢癌产生靶向治疗作用.
Abstract
Objective:To investigate the targeting ability of tubular DNA tiles modified by AS 1411 aptam-ers,HA-6AS,on ovarian cancer cells and the targeted therapy effect after loaded doxorubicin(DOX)on ovarian cancer cells.Methods:A2780 cells were incubated with AS1411 aptamers or AS-like la-beled with FAM fluorophores for 3 hours and then centrifuged to remove free HA-6(FAM-AS)or HA-6(FAM-AS-like).The fluorescence intensity of the cells was measured by flow cytometry.A2780 cells were seeded in 96-well plates and cultured overnight.Serial concentrations of HA-6AS-DOX,AS1411+DOX,and free DOX were added to 96-well plates,and CCK-8 reagent was added after 3 hours.The cell viability was calculated by measuring the absorbance under a microplate reader.Re-sults:The mean fluorescence intensity of A2780 cells incubated with HA-6(FAM-AS)was significant-ly higher than that of HA-6(FAM-AS-like)in a concentration-dependent manner(P＜0.000 1).HA-6AS-DOX could kill A2780 cells more rapidly than AS1411+DOX and free DOX within 3 hours(P＜0.01).Conclusion:AS1411 can improve the targeting of HA so that HA-6AS-DOX can have a tar-geted therapeutic effect on ovarian cancer.
关键词
适配体/卵巢癌/DNA瓦/靶向治疗Key words
Aptamer/Ovarian Cancer/DNA Tiles/Targeted Treatment引用本文复制引用
基金项目
国家自然科学基金资助项目(62172302)
国家自然科学基金资助项目(61772376)
出版年
2024
NETL
NSTL
维普
万方数据