Application of aptamer AS1411-modified tubular DNA tiles in targeted therapy of ovarian cancer
Objective:To investigate the targeting ability of tubular DNA tiles modified by AS 1411 aptam-ers,HA-6AS,on ovarian cancer cells and the targeted therapy effect after loaded doxorubicin(DOX)on ovarian cancer cells.Methods:A2780 cells were incubated with AS1411 aptamers or AS-like la-beled with FAM fluorophores for 3 hours and then centrifuged to remove free HA-6(FAM-AS)or HA-6(FAM-AS-like).The fluorescence intensity of the cells was measured by flow cytometry.A2780 cells were seeded in 96-well plates and cultured overnight.Serial concentrations of HA-6AS-DOX,AS1411+DOX,and free DOX were added to 96-well plates,and CCK-8 reagent was added after 3 hours.The cell viability was calculated by measuring the absorbance under a microplate reader.Re-sults:The mean fluorescence intensity of A2780 cells incubated with HA-6(FAM-AS)was significant-ly higher than that of HA-6(FAM-AS-like)in a concentration-dependent manner(P＜0.000 1).HA-6AS-DOX could kill A2780 cells more rapidly than AS1411+DOX and free DOX within 3 hours(P＜0.01).Conclusion:AS1411 can improve the targeting of HA so that HA-6AS-DOX can have a tar-geted therapeutic effect on ovarian cancer.
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