Effect of lncRNA HOTAIR on glioblastoma cell proliferation and its significance in clinical therapy
Objective:To investigate the biological values of lncRNA HOTAIR in glioblastoma(GBM),as well as the potential treatment drugs by bioinformatics analysis and experimental research.Methods:The sequencing profile of The Cancer Genome Atlas(TCGA)and the microarray profile of the Chi-nese Glioma Genome Atlas(CGGA)were used to evaluate the association between the HOTAIR ex-pression level and the prognosis of GBM patients,and to analyze the underlying mechanism.The HOTAIR overexpression plasmids were conducted and transfected into U251 cells.The impact of HOTAIR in GBM was verified by cell proliferation and colony formation assays.The drug sensitivity data,which were achieved from the Cancer Therapeutics Response Portal(CTRP)and PRISM Repurposing dataset,were applied to identify the candidate agents targeting HOTAIR.Results:HO-TAIR was significantly upregulated in GBM,and the higher expression of HOTAIR,the worse prog-nosis of GBM patients.Moreover,overexpressed HOTAIR could significantly enhance the prolifera-tion of U251 cells.Nine treatment agents including nicotinamide phosphoribosyltransferase inhibitor could be the target drugs for HOTAIR high expression GBM patients.Conclusion:HOTAIR was as-sociated with GBM patients'prognosis might by regulating the cell cycle process,which could be a po-tential therapeutic target.
NETL
NSTL
万方数据
维普
