LncRNA-TMEM220-AS1在肝细胞癌中的表达及对细胞增殖和侵袭能力的影响
Expression of LncRNA-TMEM220-AS1 in hepatocellular carcinoma and its effect on cell proliferation and invasiveness
任超 1叶明亮 1洪莹晖 1刘佳良 1王纯 1康辉 1丁洋 1蓝偲瑜 1常莹1
作者信息
- 1. 武汉大学中南医院消化内科/湖北省肠病临床研究中心/肠病湖北省重点实验室 湖北 武汉 430071
- 折叠
摘要
目的:分析lncRNA-TMEM220-AS1在肝细胞癌(HCC)中的表达和对HCC生物学表型的影响.方法:基于TCGA下载的数据,使用秩和检验分析TMEM220-AS1在配对及非配对癌和癌旁组织中的表达差异,Cox回归等分析方法分析TMEM220-AS1与预后的关系,使用KEGG和GO进行富集分析并通过String数据库构建与TMEM220-AS1相关的蛋白质互作网络;构建稳定敲低TMEM220-AS1的肝癌细胞株,检测TMEM220-AS1的表达量及其对HCC细胞增殖、侵袭、转移的影响.结果:HCC中TMEM220-AS1在高表达组的总体生存率显著高于低表达组,并可能通过参与凋亡相关通路发挥抑癌作用.细胞实验表明TMEM220-AS1不仅可以抑制HCC细胞的增殖,还可以显著抑制其侵袭迁移能力.结论:TMEM220-AS1在HCC中低表达,可以抑制HCC的增殖、迁移和侵袭能力,并可作为HCC的独立预后因素.
Abstract
Objective:The expression of lncRNA TMEM220-AS1 in hepatocellular carcinoma(HCC)and its function in HCC.Methods:A rank sum test was used to analyze the differences in the expression of TMEM220-AS1 in paired and unpaired cancers based on TCGA data between cancers and adjacent cancers.We used Cox regression analysis to analyze the relation between TMEM220-AS1 and prog-nosis,and by concentration analysis we constructed the protein interaction network about TMEM220-AS1.We not only detected the expression level of HCC cell lines transfected with lentivi-rus plasmid,but also analyzed its functions in HCC about proliferation,migration,and invasion.Results:The expression of TMEM220-AS1 was correlated with the overall survival rate of HCC,as the overall survival rate in HCC TMEM220-AS1 over expression group was significantly higher than in the low expression group.The expression level of TMEM220-AS1 could be used as an indepen-dent factor of HCC and could play a tumor suppressive role by participating in some metabolic path-ways.TMEM220-AS1 could significantly inhibit the proliferation,invasion,and migration of HCC.Conclusion:TMEM220-AS1 is low expressed in HCC and inhibits the proliferation and invasion of HCC.
关键词
TMEM220-AS1/肝细胞癌/生物信息学/腺病毒转染Key words
Transmembrane Protein 220-Antisense RNA 1/Hepatocellular Carcinoma/Bioin-formatics/Lenti-Virus引用本文复制引用
基金项目
国家自然科学基金(82172983)
国家自然科学基金(81670554)
武汉大学中南医院科技创新培育基金-人才培育项目(CXPY2020042)
湖北省科技厅杰出青年基金项目(2017CFA068)
国家重点研发计划(2019YFC0121505)
武汉市科技计划(2020020601012208)
出版年
2024
NETL
NSTL
万方数据