MiR-199a-3p/5p targets DUSP5/MAP3K11 to regulate fibrosis and migration of pterygium fibroblasts
Objective:To find out the function of miR-199a-3p/5p and its target gene DUSP5/MAP3K11 in human pterygium fibroblasts(HPFs).Methods:Primary HPFs were cultured from surgically ex-cised pterygium tissue using the explant method and their phenotypes were verified by quantitative real-time polymerase chain reaction(qRT-PCR).MiR-199a-3p/5p mimics and inhibitors were transfected with HPFs,and cell migration ability was determined using cell scratch assay and Transwell.Western Blot and qRT-PCR were used to detect the changes in target genes DUSP5/MAP3K11 and fibrosis markers.Immunofluorescence was used to verify the intracellular localization of epithelial-mesenchymal transition(EMT)-associated proteins.Results:Elevated EMT and fibrosis phenotype were was found in HPFs significantly as compared with that of human conjunctiva fibroblasts.MiR-NA-199a-3p/5p mimic down-regulated DUSP5/MAP3K11,and promoted HPFs'fibrosis and EMT,while miR-199a-3p/5p inhibitor up-regulated DUSP5/MAP3K11,and inhibitd HPFs'fibrosis and EMT and migration.Conclusion:MiRNA-199a-3p/5p may target DUSP5/MAP3K11 to regu-late fibrosis,EMT,and migration ability of pterygium fibroblasts.
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