miR-302a-3p targets the regulation of FGF19/GSK-3 beta/Nrf2 pathway to alleviate hypoxia/reoxygenation-induced cardiomyocyte injury
Objective:To investigate the influence of miR-302a-3p on myocardial cell injury induced by hy-poxia/reoxygenation by targeting the regulation of fibroblast growth factor 19(FGF19)/glycogen syn-thase kinase-3 beta(GSK-3β)/nuclear factor erythroid 2-related factor 2(Nrf2)pathway.Methods:H9C2 cells were separated into the control(Ctrl)group,H/R group,inhibitor negative control(NC)group,miR-302a-3p inhibitor group,miR-302a-3p inhibitor+si-NC group,and miR-302a-3p inhibi-tor+si-FGF19 group.The levels of miR-302a-3p expression,cell proliferation(OD450),apoptosis,re-active oxygen species(ROS),superoxide dismutase(SOD),malondialdehyde(MDA)levels and FGF19,p-GSK-3β,nuclear Nrf2,Bcl-2-related X protein(Bax),and cysteine protease 3(caspase-3)protein expression were detected,and the relationship between miR-302a-3p and FGF19 was ex-plored.Results:Compared with those in the Ctrl group,the miR-302a-3p,apoptosis rate,ROS,MDA levels,Bax,caspase-3,p-GSK-3β protein expression in H9C2 cells in the H/R group in-creased,the FGF19 and nuclear Nrf2 protein expression,OD450 value,and SOD level decreased(P<0.05);Compared with those the inhibitor NC group,the changing trend of the corresponding indica-tors in the miR-302a-3p inhibitor group was opposite to the above(P<0.05);si-FGF19 attenuated the protective effect of down-regulated miR-302a-3p against H/R-induced cardiomyocyte injury.Conclu-sion:Down-regulation of miR-302a-3p can alleviate H/R-induced cardiomyocyte injury by targeting the activation of FGF19/GSK-3β/Nrf2 pathway.
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