Protective effect of Panax notoginseng saponins on hippocampal neurons of epileptic rats by regulating miR-204
Objective:To explore the effects of total saponin of Panax notoginseng leaves(TSPNL)on miRNA-204 and hippocampal neuron damage in epileptic(EP)rats.Methods:A total of 75 SD rats were randomly divided into the blank control group,EP group,TSPNL group,TSPNL+ Antagomir-NC group,and TSPNL+Antagomir-miR-204 group,with 15 rats in each group.EP rat model was established by lithium-pilocarpine.After the corresponding interventions were given,the behavioral changes of rats were observed;Electroencephalogram(EEG)was used to monitor the changes of EEG;RT-qPCR was used to detect the expression of miR-204,tropomyosin-related ki-nase B(TrkB),interleukin-1β(IL-1β),and interleukin-6(IL-6)mRNA in the hippocampus;HE stain-ing was used to detect the pathological changes of the hippocampus;immunofluorescence(IF)was used to detect the number of neuron-specific nuclear protein(NeuN)positive cells in hippocampus;flow cytometry was used to detect the apoptosis rate of hippocampal neurons;Western blot was used to detect the expression of Bcl-2,Bax and Caspase-3 in the hippocampus.Results:Compared with those in the EP group,the Racine score,the number and duration of seizures in 90 minutes,the num-ber of spike and slow waves,the expression of TrkB mRNA,IL-1β mRNA,and IL-6 mRNA in the hippocampus,the apoptosis rate of neurons,the expression of Bax and Caspase-3 in TSPNL group and TSPN +Antagomir NC group were lower(P<0.05),the expression of miR-204 in hippocampal tissues,the number of NeuN positive cells and the expression of Bcl-2 in hippocampal CA1 and CA3 were higher(P<0.05),the damage of hippocampal CA1 and CA3 neurons was significantly alleviat-ed;the antagonist of miR-204 can significantly aggravate the damage of hippocampal neurons and re-verse the anti-EP effect of TSPNL.Conclusion:TSPNL may protect hippocampal neurons from EP injury by up-regulating the expression of miR-204.
Total Saponin of Panax Notoginseng LeavesEpilepsyHippocampal NeuronsApoptosisMicroRNA-204
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