Long non-coding RNA NORAD accelerates the progression of lung adenocarcinoma via promoting epithelial-mesenchymal transition
Objective:To investigate the role of long non-coding RNA(lncRNA)NORAD in lung adeno-carcinoma(LUAD)development and its potential mechanism.Methods:By analyzing the expres-sion of NORAD in 19 cases of lung adenocarcinoma and adjacent normal tissues,we demonstrated the relationship between NORAD expression and clinicopathologic stage.The expression of NORAD in A549 and NCI-H1299 cells was detected by RT-PCR.si-NORAD interference silenced A549 and NCI-H1299 cells,and the effects of si-NORAD on cell proliferation,migration and invasion were de-tected.Western Blot was used to detect the expression of MMP9,MMP2,Vimentin,N-Cadherin,and E-Cadherin;based on the TCGA database,LUAD transcriptome data were extracted for Weight-ed Correlation Network Analysis(WGCNA).Further Gene Set Enrichment Analysis(GSEA)was used to analyze the possible signaling pathways of NORAD promoting LUAD,and then Western Blot was used to validate the effect.Results:NORAD was highly expressed in lung adenocarcinoma and prone to lymph node metastasis.NORAD was significantly overexpressed in A549 and NCI-H1299 cells,and the knockdown of NORAD significantly attenuated the proliferation,migration,and invasion of A549 and NCI-H1299 cells.Knockdown of NORAD up-regulated the expression of MMP9,MMP2,Vimentin,and N-Cadherin,and down-regulated the expression of E-Cadherin.The results of WGCNA,GSEA,and Western Blot experiments showed that NORAD promoted the development of LUAD through the PI3K/AKT signaling pathway.Conclusion:Overexpression of NORAD in LUAD could lead to a poor prognosis for LUAD patients.NORAD could affect the ge-nome stability of LUAD cell lines through PI3K-AKT-mTOR transduction,thereby promoting the occurrence and metastasis of LUAD.
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