Effect of mechanical force on the expression of Piezo1 and apoptosis of fibroblast in female anterior vaginal wall and their correlation analysis
Objective:To explore the effect of mechanical force on the expression of Piezo1 and apoptosis of fibroblast in the female anterior vaginal wall,and to analyze the correlation between mechanical force and apoptosis of stress urinary incontinence(SUI).Methods:A total of 43 patients who under-went surgical treatment in the Department of Gynecology of Renmin Hospital of Wuhan University from March 2020 to December 2021 were selected,including 23 patients with SUI(anterior wall pro-lapse or cystocele,POP-Q Ⅲ-Ⅳ:Ba＞+1),and the remaining 20 patients(control group)under-went total hysterectomy for benign tumors.The enzyme digestion method was used to obtain primary fibroblasts from the female anterior vaginal wall,which was identified by immunostaining.Anterior vaginal wall fibroblasts in the control group patients periodically explored the four-point bending cell mechanical loading system(mechanical loading group),and the mechanical parameters were set to 2666 με(loading displacement was 2 mm).The immunofluorescence staining and flow cytometry were adopted respectively to assay the expression of Piezo1 and apoptosis,and the correlation of the ex-pression of Piezo1 and apoptosis of fibroblasts was studied.Results:The expression of Piezo1 in fi-broblasts of the SUI group was higher than that of the control group,and the apoptosis was higher than that of the control group.The apoptosis rate and Piezo1 protein expression of fibroblasts in the control group increased after periodic mechanical force was applied.Correlation analysis showed that Piezo1 protein expression was positively correlated with the apoptosis of fibroblasts in SUI group and the mechanical loading group.Conclusion:Excessive mechanical stimulation could induce SUI.Both Piezo1 protein and apoptosis may be involved in this process,and Piezo1 protein may participate in the process of SUI by inducing fibroblast apoptosis.
Stress Urinary IncontinencePiezo1ApoptosisMechanical Force
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