机械力刺激对女性阴道前壁成纤维细胞Piezo1蛋白表达和凋亡的影响及其相关性分析
Effect of mechanical force on the expression of Piezo1 and apoptosis of fibroblast in female anterior vaginal wall and their correlation analysis
李洋 1廖文欣 1刘婷婷 1洪莉1
作者信息
- 1. 武汉大学人民医院妇科 湖北 武汉 430060
- 折叠
摘要
目的:探讨过度的机械力刺激对人阴道前壁成纤维细胞Piezo1蛋白表达及凋亡的影响,并分析两者在机械力诱导压力性尿失禁(SUI)中的相关性.方法:选取2020年3月-2021年12月于武汉大学人民医院妇科行手术治疗的患者43例,其中SUI患者23例(伴前壁脱垂或膀胱膨出,POP-Q Ⅲ～Ⅳ:Ba＞+1),其余20例为因良性肿瘤行子宫全切手术的患者(对照组),取阴道前壁组织,采取改良酶消化法获得人阴道前壁成纤维原代细胞,并进行成纤维细胞鉴定.另取对照组患者阴道前壁成纤维细胞采用四点弯曲细胞力学加载系统进行周期性力加载(加力组),力学参数设置为2 666 µε(加载位移为2 mm),分别采用免疫组化染色及流式细胞术检测对照组、SUI组、加力组患者的阴道前壁成纤维细胞的Piezo1蛋白表达和细胞凋亡的情况,并对3组细胞的Piezo1蛋白表达和凋亡进行相关性分析.结果:SUI组患者的成纤维细胞Piezo1蛋白表达高于对照组(P＜0.000 1),细胞凋亡率高于对照组(P＜0.000 1);对照组患者成纤维细胞加载周期性机械力后其细胞凋亡率增加(P＜0.000 1),Piezo1蛋白表达上升(P＜0.000 1).相关性分析发现,SUI组和加力组的Piezo1蛋白表达和细胞凋亡呈正相关(r2=0.611 6,P＜0.000 1;r2=0.464 2,P=0.000 9).结论:过度的机械力刺激可诱导SUI的发生,Piezo1蛋白和细胞凋亡可能均参与了这一过程,且Piezo1蛋白可能通过诱导的细胞凋亡参与机械力引起SUI的进程.
Abstract
Objective:To explore the effect of mechanical force on the expression of Piezo1 and apoptosis of fibroblast in the female anterior vaginal wall,and to analyze the correlation between mechanical force and apoptosis of stress urinary incontinence(SUI).Methods:A total of 43 patients who under-went surgical treatment in the Department of Gynecology of Renmin Hospital of Wuhan University from March 2020 to December 2021 were selected,including 23 patients with SUI(anterior wall pro-lapse or cystocele,POP-Q Ⅲ-Ⅳ:Ba＞+1),and the remaining 20 patients(control group)under-went total hysterectomy for benign tumors.The enzyme digestion method was used to obtain primary fibroblasts from the female anterior vaginal wall,which was identified by immunostaining.Anterior vaginal wall fibroblasts in the control group patients periodically explored the four-point bending cell mechanical loading system(mechanical loading group),and the mechanical parameters were set to 2666 με(loading displacement was 2 mm).The immunofluorescence staining and flow cytometry were adopted respectively to assay the expression of Piezo1 and apoptosis,and the correlation of the ex-pression of Piezo1 and apoptosis of fibroblasts was studied.Results:The expression of Piezo1 in fi-broblasts of the SUI group was higher than that of the control group,and the apoptosis was higher than that of the control group.The apoptosis rate and Piezo1 protein expression of fibroblasts in the control group increased after periodic mechanical force was applied.Correlation analysis showed that Piezo1 protein expression was positively correlated with the apoptosis of fibroblasts in SUI group and the mechanical loading group.Conclusion:Excessive mechanical stimulation could induce SUI.Both Piezo1 protein and apoptosis may be involved in this process,and Piezo1 protein may participate in the process of SUI by inducing fibroblast apoptosis.
关键词
压力性尿失禁/Piezo1/细胞凋亡,机械力Key words
Stress Urinary Incontinence/Piezo1/Apoptosis/Mechanical Force引用本文复制引用
基金项目
国家自然科学基金青年基金(82301828)
中央高校基本科研业务费专项(2042022kf1110)
国家重点研发计划(2018YFC2002204)
国家重点研发计划(2021YFC2701302)
国家自然科学基金面上项目(81971364)
出版年
2024
NETL
NSTL
万方数据