Exploring the mechanism of liver injury in type 1 diabetes based on transcriptome sequencing technology
Objective:To analyze the mRNA expression in the liver tissue of streptozotocin(STZ)-induced liver injury in type 1 diabetic rats and control rats,identify the hub genes and discuss the role of peroxi-some proliferator-activated receptors(PPARs)signaling pathway in this model.Methods:A total of twenty SD male rats were randomly divided into the model group and control group.The model group received a single intraperitoneal injection of 60 mg/kg of STZ while the control group received the same amount of dissolved solution.The fasting blood glucose levels and liver pathology were mea-sured to assess the success of the modeling on the third day.Then we searched for differentially ex-pressed genes in rat liver tissues by RNA-seq,performed functional enrichment of differentially ex-pressed genes,and mapped protein interaction networks.Hub genes were acquired by MCC,MNC,and DMNC algorithms.Results:Compared to the control group,fasting blood glucose was signifi-cantly higher in the model rats,and pathological staining of the liver(HE stain and Oil Red O stain)showed mild steatosis.We then screened the liver tissue for differential genes based on |log2FC|＞1 and P＜0.05.We identified 298 genes that were highly expressed and 212 genes that were lowly ex-pressed in the model group as compared with those in the control group.Enrichment analysis by KEGG,GO and GOKEGG,and log2FC(P.adj＜0.1)revealed that in addition to the 19 signaling pathways identified by KEGG analysis,we found 282 signaling pathways enriched in cellular compo-nents,26 signaling pathways enriched in bioprocesses,and 9 pathways enriched in molecular func-tions.Eight hub genes Top2a,Kif11,Plk1,Ttk,Bub1,Cdk1,Ccna2,and Cdc20 can be acquired by taking the intersection of the three gene sets obtained by the three algorithms of MCC,MNC and,DMNC.Conclusion:The differentially expressed genes in the STZ-induced liver injury in type 1 dia-betic rats were screened by RNA-seq,and it was found that PPARs signaling may play a role in this disease,and the precise mechanism needs to be further clarified.
Type 1 DiabetesStreptozotocinLiver InjuryPeroxisome Proliferator-Activated Receptors(PPARs)RNA-seq
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