高浓度4NQO建立小鼠舌癌动物模型的改进方法
An improved method for the establishment of the high concentration of 4NQO induced mouse tongue cancer model
艾克拜尔·托乎提 1林泽华 1蔡捷 1陈雄1
作者信息
- 1. 武汉大学中南医院耳鼻咽喉头颈外科 湖北 武汉 430071
- 折叠
摘要
目的:利用含高浓度4-硝基喹啉-1-氧化物(4NQO)的水与固体饲料相结合方式,旨在探讨短时间构建高生存率舌癌小鼠模型的方法.方法:将12只BALB/c小鼠随机分为对照组(H2O,n=4)、中剂量实验组(250 µg/mL 4NQO,n=4)和高剂量实验组(300 µg/mL 4NQO,n=4),固液交替喂养,对小鼠的外貌、体质量和舌黏膜状态进行观察并记录,12周后处死小鼠,取材进行HE染色.结果:研究发现中剂量组的小鼠均造模成功,其中3只小鼠的舌黏膜出现乳头状样新生物,并且HE染色发现癌巢及角化珠的病理特征,余1只小鼠舌黏膜仅出现白斑样改变,病理表现为舌黏膜上皮重度异型增生.而高剂量组中的小鼠在造模过程中全部死亡.结论:250 μg/mL4NQO能在12周内成功诱导出舌癌小鼠模型,并能保证较高的生存率.
Abstract
Objective:To investigate an efficient and reliable method for establishing a mouse model of tongue cancer within a short time while maintaining a high survival rate,through a new approach of combined drinking water which contains a high concentration of 4-nitroquinoline-1-oxide(4NQO)and 4NQO-medicated feed.Methods:Twelve BALB/c mice were randomly divided into a control group(H2O,n=4)and two experimental groups,which included a medium dose group(250 μg/mL 4NQO,n=4)and a high dose group(300 μg/mL 4NQO,n=4).The body weight and tongue mucosa status were recorded.The mice were fed with 4NQO water and/or 4NQO-medicated feed for 10 weeks and were executed at the end of 12 weeks,and tongue mucosa samples were taken for HE staining and pathological observation.Results:Successful modeling of tongue cancer was observed in the mice of the medium-dose group,with three of them showing papillary neoplasia in the tongue mucosa.HE staining revealed the presence of nests and keratinized beads.One mouse from the same group exhibited only leukoplakia-like changes in the tongue mucosa,and the epithelium of tongue mucosa dis-played significantly heterogeneous.All mice in the high-dose group died during the experiment.Con-clusion:The administration of 250 μg/mL 4NQO,combined with medicated feed,enabled the induc-tion of a tongue cancer model in mice within 12 weeks while maintaining a high survival rate.
关键词
舌癌/动物模型/4-硝基喹啉十氧化物Key words
Tongue Cancer/Animal Model/4-Nitroquinoline-1-Oxide引用本文复制引用
基金项目
国家自然科学基金面上项目(82071033)
出版年
2024
NETL
NSTL
万方数据