Expression,significance,and molecular mechanisms of myosin 10 in head and neck squamous cell carcinoma based on Oncomine and TCGA database analysis
Objective:To investigate the expression,significance,and molecular mechanisms of the myosin 10(MYO10)gene in patients with head and neck squamous cell carcinoma(HNSCC)based on the can-cer genome atlas(TCGA)and Oncomine database.Methods:The limma R package and univariable Cox analysis were used,and genes with differential expression and prognostic value were identified in the TCGA cohort,Oncomine database,and Kaplan-Meier survival online analysis.Gene set enrich-ment analysis(GSEA)was applied to explore the potential mechanisms of MYO 10 in HNSCC.The MYO10 protein expression was assessed by the Clinical Proteomic Tumor Analysis Consortium(CPTAC)and the Human Protein Atlas.The relationship between MYO10 mRNA expression and immune infiltrates was determined by estimation of stromal and immune cells in malignant tumour tis-sues using expression data(ESTIMATE).The relationship of MYO10 expression to the Intel genome heterogeneity and burst changes view was explored using the Maftools and ComplexHeatmap R pack-age.Results:A total of 519 HNSCC cancer tissue samples and 44 adjacent non-cancerous tissue sam-ples in the TCGA database were analyzed,and MYO10 was ranked high among HNSCC differential genes with significantly high expression(P＜0.000 1).Analysis of MYO10 expression on 9 HNSCC studies in the Oncomine database showed that MYO10 was significantly up-regulated in HNSCC tu-mor tissues compared with non-tumor tissues(P＜0.05).Additionally,MYO10 overexpression was significantly correlated with poor overall survival(OS)in HNSCC patients.The correlation analysis of immune infiltration showed that MYO 10 was significantly correlated with multiple immune cell infiltra-tion.GSEA analysis revealed the potential functions of MYO10 in activating focal adhesion kinase(FAK),regulating cytoskeletal protein,oxidative phosphorylation,and Wnt signaling pathways.Conclusion:MYO 10 was implicated as a potential prognostic biomarker and also provided novel in-sights into the mechanisms underlying MYO10-mediated unfavorable outcomes in HNSCC patients.
Head and Neck Squamous Cell CarcinomaMyosin 10OncomineTCGA
NETL
NSTL
万方数据
