Neuroprotective mechanism of interferon regulatory factor 4 mediating therapeutic hypothermia in mice with ischemic stroke
Objective:To explore the effect and mechanism of interferon regulatory factor 4(IRF4)mediating therapeutic hypothermia(TH)on ischemic stroke(IS)in mice.Methods:TH was performed on wild-type and IRF4 knockout-type IS mice.After TH,the expression levels of pro-inflammatory cytokines and anti-inflammatory cytokines,M1 and M2 macrophage markers,and IRF4 in ischemic penumbra brain tis-sue were detected.Neural function was tested,the cerebral infarction volume was calculated after TTC staining,and the apoptosis was assayed with immunofluorescent staining.Results:TH significantly re-duced cerebral infarction volume and improved neurological function of IS mice.IRF4 knockout elimi-nated the protective effect of TH:TH neither reduced the volume of cerebral infarction nor improved the neurological function of IRF4 knockout IS mice.Conclusion:TH may regulate the macrophage polariza-tion and expression of inflammatory cytokines through up-regulating the expression of IRF4 and finally exert anti-inflammatory and brain protective effects.
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