Effect of anti-CD20 monoclonal antibody on airway inflammation in a murine model of asthma
objective:To investigate the effect of anti-CD20 monoclonal antibody(mAb)on airway inflam-mation in a murine model of asthma.Methods:Fifteen female BALB/c mice aged 6-8 weeks were randomly divided into the normal control group,asthma control group,and anti-CD20 mAb treatment group,with 5 mice in each group.The asthma group and the anti-CD20 mAb treatment group were sensitized and challenged with house dust mite(HDM),while the normal control group was replaced with an equivalent dose of PBS.The anti-CD20 mAb was given by intranasal drip combined with in-traperitoneal injection two days before the first challenge in the anti-CD20 mAb treatment group,and an equal amount of IgG2c isotype control was given instead in the normal control group and asthma group.Hematoxylin-eosin(HE)staining and periodic acid-schiff(PAS)staining were used to assess lung histopathology and airway goblet cell hyperplasia.Total and classified counts of cells in bronchial alveolar lavage fluid(BALF)analyzed by Wright-Giemsa staining.Enzyme-linked immunosorbent as-say(ELISA)was adopted for the detection of serum HDM-specific IgE,IL-4,IL-5,IL-13,IFN-γ,and IL-10 levels in BALF.The number of CD19+B cells in the lungs was detected by flow cytome-try.Results:Compared with those in the asthma group,lung tissue inflammatory cell infiltration and the number of airway goblet cells in asthmatic mice in the anti-CD20 mAb treatment group were significantly reduced,as well as the total number of cells,eosinophils,macrophages,and lymphocytes in BALF(P<0.01);serum HDM-specific IgE and IL-4,IL-5 and IL-13 levels in BALF were signifi-cantly reduced(P<0.01);no statistically significant changes was found in IFN-γ levels in BALF(P>0.05);and IL-10 levels in BALF were significantly increased(P<0.01).Meanwhile,the number of lung CD19+B cells in asthmatic mice in the anti-CD20 mAb treatment group was significantly reduced(P<0.01).Conclusion:The combination of intranasal drip and intraperitoneal injection of anti-CD20 mAb attenuates airway inflammation in a murine model of asthma,which might be associated with in-creased airway IL-10 level.
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