IGF-1R inhibitor inhibits corneal stromal fibrosis after alkali burn in mice by regulating autophagy
Objective:To investigate the expression of insulin-like growth factor 1 receptor(IGF-1R)in mice cornea after alkali burn,and to explore the effect and mechanism of IGF-1R inhibitor(PPP)on corneal fibrosis.Methods:Eighty-one mice were randomly divided into the control group,alkali burn group(AB),alkali burn+solvent(DMSO)group,and alkali burn+IGF-1R inhibitor(PPP)group.Ex-cept in the control group,the alkali burn models were established in the other three groups.From one day before establishing the model,mice in PPP the group were given 2 mol/L PPP eye drops,and those in the DMSO group were given DMSO eye drops at the same dose for 14 d.PCR and Western blot were used to detect the levels of IGF-1R mRNA and protein in mice cornea after alkali burn.The slit lamp microscope was used to observe corneal opacity after the intervention of IGF-1R inhibition.HE staining was used to observe the pathological injury in corneal tissue.PCR,Western Blot,and immunofluorescence staining were used to evaluate the expression of α smooth muscle actin(α-SMA)and LC3 in corneal tissue.Results:At the end of the 14th day after alkali burn,there was an in-creased trend in the levels of IGF-1R mRNA and protein of the AB group as compared with the con-trol group.Compared with those in the DMSO group,there were decreased corneal transparency and pathological injury,lower expression levels of α-SMA mRNA and protein,as well as higher expres-sion levels of LC3 mRNA and protein in the PPP group.Conclusion:IGF-1R inhibition may facili-tate autophagy and inhibit corneal stromal fibrosis to relieve corneal injury after alkali burn.
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