Determination of voriconazole concentration in serum of children with blood diseases by high-performance liquid chromatography and its clinical application
Objective:To establish a high-performance liquid chromatography(HPLC)method for the deter-mination of voriconazole(VRC)in human serum,which can rapidly,accurately,and stably monitor the concentration of voriconazole in children with blood diseases and lay a foundation for guiding clini-cal drug use.Methods:Reverse phase HPLC method was used(Chromatographic column:Eclipse XDB-C18 column,4.6 mm×150 mm,5 µm.Mobile phase:Ammonium formate aqueous solution(pH 4.0):acetonitrile=66:34;Flow rate:1.0 mL/min;Wavelength:256 nm).The blood samples were pretreated with Waters Sep Pak C18 solid phase extraction column.Results:The retention time of VRC and internal standard peaks was 9.7 min and 11.0 min,respectively,with good separation and without interference from endogenous substances in blood samples.A regression equation was Y=0.295 3X-0.004 3,R2=0.999,the linear range of VRC in serum was 0.10-19.87 μg/mL,the lower limit of quantification was 0.10 μg/mL;RSDs of accuracy,precision and stability were<5％.Blood samples were monitored in 6 patients.Conclusion:The concentration of voriconazole in the blood varies significantly among children.Monitoring therapeutic drugs in children with blood diseases is helpful to improve clinical efficacy and reduce the risk of adverse reactions.This method is simple,accurate,and suitable for clinical monitoring of voriconazole.
VoriconazoleHigh-Performance Liquid ChromatographyTherapeutic Drug MonitoringChildrenHematologic Malignancies
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