Expression and function of transcription factor PU.1 in human gliomas
Objective:To investigate the expression character of the transcription factor PU.1 in human gliomas and analyze its correlation with survival of patients and tumor vascularization.Methods:We collected 4 non-tumor human brain samples and 23 human glioma samples,including 6 in WHO-Ⅱgrade,8 in WHO-Ⅲ grade,and 9 in WHO-Ⅳ grade.We analyzed the expressing location of PU.1 and Iba1 with immunohistological methods and clarified the correlation between the number of PU.1 positive cells,glioma grades and the number of endothelial cells.With TCGA glioma data,we ana-lyzed the correlation between the transcription levels of SPI1(the gene encoding PU.1)and glioma grades,as well as the correlation between the transcription levels of AIF1(the gene encoding Iba1)and the survival time of patients.Results:The expression of PU.1 colocalized with Iba1,the specific marker of tumor-associated macrophages.The number of PU.1 positive cells per field was 1.79±0.30 in non-tumor human brain tissue,18.56±2.01 in WHO-Ⅱgrade gliomas,25.35±3.98 in WHO-Ⅲ grade gliomas,and 31.40±2.74 in WHO-Ⅳ grade gliomas.Also,50.36±5.92 endothe-lial cells per field were counted in gliomas with more PU.1 positive cells,while 33.29±3.73 endo-thelial cells per field in gliomas with less PU.1 positive cells.By analyzing TCGA data,we found the transcription counts of SPI1 had a linear correlation to the transcription counts of AIF1 in human glio-mas,and higher transcription counts of SPI1 predicted worse survival.Conclusion:PU.1 is a specific transcription factor for tumor-associated macrophages in human gliomas.The expression of PU.1 cor-relates with the grades of gliomas,and it is a potential molecular predictor for the survival of patients,which might play an important role in modulating tumor-associated macrophages to promote glioma-angiogenesis.Further exploration of the function of PU.1 on glioma-vascularization will give rise to new ideas for novel brain tumor treatment.
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