Piezo1 boosts the epithelial-mesenchymal transformation process in ovarian cancer
Objective:To analyze the expression and correlation of Piezo1 expression and the related pro-teins of tumor epithelial-mesenchymal transformation(EMT)in the tissues of ovarian cancer patients and to investigate the influence of Piezo1 on the EMT process of ovarian cancer cells(SKOV3 and A2780).Methods:The ovarian cancer-related signaling pathways activated by Piezo1 were analyzed by the bioinformatics GSEA method.Immunohistochemistry and Western Blot were used to detect the expressions of Piezo1 protein and the EMT pathway-related proteins E-cadherin and N-cadherin in normal ovarian and ovarian cancer tissues.The correlation between Piezo1 expression and the expres-sion of E-cadherin and N-cadherin was analyzed.Ovarian cancer cells(SKOV3 and A2780)were treated with Yoda1 10 μmol/L for two days.According to the transfection of lentivirus,the ovarian cancer cells(SKOV3 and A2780)were divided into sh-NC(transfection of sh-NC lentivirus)and sh-Piezo1(Transfection of sh-Piezo1 lentivirus)groups.Then Western Blot was used to detect the ex-pression of E-cadherin and N-cadherin in SKOV3 and A2780 cells of each group.Results:The pro-tein expression level of Piezo1 was significantly higher in ovarian cancer than in normal ovarian tissue(P<0.05),and it was found that Piezo1 significantly activated the EMT signaling pathway in ovarian cancer by bioinformatics GSEA enrichment analysis(P<0.01).Western Blot and immunohistochemi-cal results showed that the protein level of E-cadherin in ovarian cancer tissues was significantly de-creased(P<0.05),while the expression level of N-cadherin was significantly increased(P<0.05).Correlation analysis showed that Piezo1 expression in ovarian cancer was significantly negatively corre-lated with that of E-cadherin(r=-0.731 7;P=0.001 5),and positively correlated with that of N-cadherin(r=0.600 7;P=0.003).In SKOV3 and A2780 cells treated with Yoda1,the expression level of E-cadherin was decreased(both P<0.05),while the expression level of N-cadherin was sig-nificantly increased(P<0.05 and P<0.01,respectively).After Piezo1 knockdown in SKOV3 and A2780 cells,the expression level of E-cadherin was significantly increased(P<0.01 and P<0.001,respectively),and that of N-cadherin was decreased(P<0.05 and P<0.01,respectively).Conclu-sion:The high Piezo1 expression may be related to poor prognosis of patients,and may also play a role in the development of ovarian cancer by promoting the process of EMT.
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