Function of TIGD1 in poorly differentiated nasopharyngeal squamous cell carcinoma cell lines
Objective:To investigate the function of trigger transposable element-derived 1(TIGD1)in poorly differentiated nasopharyngeal squamous cell cancer cells.Methods:siRNAs and overexpressed plasmids were used to interfere TIGD1 gene in CNE2 and HNE1 cell lines.The gene knockdown and overexpression efficiency were verified by real-time quantitative PCR and Western Blot.CCK-8 method was used to measure the proliferation activity of cells in different treatment groups.The apop-tosis rate and cell cycle changes in different treatment groups were detected by real-time fluorescence quantitative PCR,Western Blot,and flow cytometry.Results:In CNE2 and HNE1 cell lines,TIGD1 knockdown slowed cell proliferation and increased cell apoptosis rate,while the cell cycle was arrested.The overexpression of the TIGD1 gene accelerated the cell proliferation,decreased the cell apoptosis rate,and accelerated the cell proliferation.Conclusion:The high expression of TIGD1 in poorly differentiated nasopharyngeal squamous cell cancer may be related to the occurrence and pro-gression of the disease,which may provide clues for finding its therapeutic targets.
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