Inhibition of taurine upregulation gene 1 reduces cerebral ischemia-reperfusion injury in rats by upregulating brain-derived nerve growth factor
Objective:To investigate the role of taurine upregulated gene 1(TUG1)in cerebral ischemia-re-perfusion injury(CIRI)in rats and its possible mechanism.Methods:A CIRI rat model was estab-lished and transfected with antisense oligonucleotides(ASO)-TUG1 and its blank control ASO-NC.All rats were divided into the control group,CIRI group,CIRI+ASO-NC group,and CIRI+ASO-TUG1 group.The modified neurological severity score(mNSS)was used to evaluate the neurological function of rats in each group.TTC staining was used to observe the volume of cerebral infarction in rats.The expression levels of TUG1 and brain-derived neurotrophic factor(BDNF)mRNA were de-tected by PCR.Western Blot and immunohistochemistry experiments were used to detect the expres-sion of BDNF protein in each group.Results:The expression level of TUG1 in the brain tissue of rats in the CIRI group was significantly higher than that in the control group(P＜0.05).In addition,ASO-TUG1 significantly reduced the expression level of TUG1(P＜0.05).The mNSS score and cerebral infarction volume of rats in the CIRI+ASO-TUG1 group were significantly lower than those in the CIRI group and CIRI+ASO-NC group(P＜0.05).The expression levels of BDNF mRNA and pro-tein in the brain tissue of rats in the CIRI+ASO-TUG1 group were higher than those in the CIRI group and the CIRI+ASO-NC group(P＜0.05).Conclusion:Downregulating the expression of TUG1 can significantly increase BDNF mRNA and protein expression levels in the brain tissue of CIRI rats,reduce the volume of cerebral infarction,and improve the rats'neurological function.
Taurine Upregulates Gene 1Cerebral Ischemia-Reperfusion InjuryBrain-De-rived Neurotrophic FactorNeurological Function
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