基于数据挖掘分析UHRF1在肺腺癌中的表达及临床意义
Expression of UHRF1 in lung adenocarcinoma by data mining and its clinical significance
曾榆凯 1王光辉 2郑昊天 1项云治 1柳勇 1马国元 2杜贾军2
作者信息
- 1. 山东大学 山东 济南 250012
- 2. 山东大学附属省立医院 山东 济南 250021
- 折叠
摘要
目的:旨在基于生物信息数据库进行数据挖掘以探索泛素样含PHD和环指域1(UHRF1)在肺腺癌中的表达情况并分析其对肺腺癌患者的预后影响.方法:使用GEPIA数据库探索UHRF1在泛癌中的表达情况.应用UALCAN数据库和Kaplan-Meier Plotter数据库分析UHRF1和肺腺癌患者的临床相关性及预后的意义.通过GSEA分析UHRF1的相关功能.在String数据库中分析与UHRF1相互作用的蛋白.运用TCIA数据库分析肺腺癌中UHRF1表达水平和免疫浸润的关系.结果:UHRF1在大部分癌症存在表达差异,肺腺癌组织中UHRF1表达水平和正常组织相比显著升高(P＜0.05). UHRF1表达量和肺癌患者的年龄(P＜0.05)、性别(P＜0.05)、吸烟习惯(P＜0.05)、病理分期(P＜0.05)显著相关.Kaplan-Meier曲线表明UHRF1高表达和不良预后相关(P＜0.05).GSEA、蛋白-蛋白相互作用和免疫浸润分析表明UHRF1可能作为一个潜在的分子生物标志物,通过影响肺腺癌细胞的增殖、迁移、DNA甲基化和肿瘤免疫浸润,从多个方面促进肺腺癌的发生发展.结论:UHRF1可能作为肺腺癌预后的生物标志物,具有重要的临床价值.
Abstract
Objective:To explore the expression of UHRF1(Ubiquitin-like with PHD and RING finger do-main 1)in lung adenocarcinoma and analyze its prognostic impact on lung adenocarcinoma patients by conducting data mining based on bioinformatic databases.Methods:The expression of UHRF1 in pan-cancer was explored using the GEPIA database.The UALCAN and Kaplan-Meier Plotter data-base were used to analyze the clinical correlation and prognostic significance of UHRF1 in lung adeno-carcinoma patients.The related functions of UHRF1 were analyzed by GSEA.Proteins interacting with UHRF1 were analyzed in the String database.The relationship between the level of UHRF1 ex-pression and immune infiltration in lung adenocarcinoma was analyzed using the TCIA database.Results:The expression of UHRF1 was different in pan-cancers,and the expression level of UHRF1 in lung adenocarcinoma tissues was significantly higher than that in normal tissues(P＜0.05).The ex-pression of UHRF1 was significantly correlated with age,gender,smoking habits,and pathological stages of lung cancer patients.Kaplan-Meier curves indicated that high UHRF1 expression was associ-ated with poor prognosis(P＜0.05).GSEA,protein-protein interaction(PPI)and immune infiltration analysis suggest that UHRF1 may serve as a potential molecular biomarker and promote lung adeno-carcinoma by DNA methylation,ubiquitination,proliferation,migration and tumor immune infiltra-tion.Conclusion:UHRF1 may serve as a prognostic biomarker for lung adenocarcinoma and has im-portant clinical significance.
关键词
UHRF1/肺腺癌/Kaplan-Meier分析/基因集富集/蛋白-蛋白相互作用/免疫浸润Key words
UHRF1/Lung Adenocarcinoma/Kaplan-Meier Analysis/Gene Set Enrichment/Protein-Protein Interaction/Immunoinfiltration引用本文复制引用
基金项目
山东省自然科学基金面上项目(ZR2021MH192)
山东省自然科学基金面上项目(ZR2020QH215)
出版年
2024
NETL
NSTL
万方数据