DNASE1L3 expression and its impact on prognosis in hepatocellular carcinoma based on bioinformatics
Objective:To analyze the expression of DNASE1L3 in hepatocellular carcinoma and its impact on patient prognosis,and explore the potential pathways through bioinformatics analysis.Methods:Based on TCGA,GSE14520,and HPA databases,the differential expression of DNASE1L3 be-tween normal and tumor tissues in hepatocellular carcinoma was analyzed.Univariate and multivariate COX regression analysis and Kaplan-Meier survival analysis were conducted to explore the impact of DNASE1L3 on the prognosis of hepatocellular carcinoma.The expression changes of DNASE1L3 in different stages of tumor progression were analyzed based on GSE6764 data.The relationship be-tween DNASE1L3 and immune cell infiltration,stromal cell infiltration,and tumor purity was ana-lyzed using the ESTIMATE algorithm.The enriched results of GSVA were subjected to differential analysis using the Limmapackage,and the co-expressed genes of DNASE1L3 were explored through the GeneMINIA online tool.Results:DNASE1L3 was downregulated in tumor tissues,which was associated with poor prognosis in patients.The expression of DNASE1L3 was decreased in larger tu-mors,higher alpha fetoprotein(AFP)levels,and more advanced stages of tumor tissue,and it further reduced tumor progression.Low expression of DNASE 1L3 may indicate lower immune stromal abun-dance and higher tumor purity in the tumor microenvironment,and it was associated with increased ex-pression of some immune checkpoint genes.Enrichment differential analysis and co-expression gene enrichment suggested that DNASE1L3 was mainly involved in DNA damage repair,cell cycle,fatty acid metabolism,and glycolysis,and it may be involved in regulating immune cell infiltration in hepa-tocellular carcinoma.Conclusion:DNASE1L3 was downregulated in hepatocellular carcinoma tis-sues and was involved in the tumor progression and tumor immune processes.It is a promising prog-nostic molecular biomarker and therapeutic target that requires further exploration and investigation.
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