Transcriptomic analysis in a mouse model of magnetic beads-induced chronic intraocular hypertension glaucoma
Objective:To analyze the alterations in retinal differential expression genes(DEGs)and signaling pathways in a mouse model of chronic intraocular hypertension glaucoma induced by magnetic beads,and to utilize transcriptomic sequencing(RNA-seq)and bioinformatics to elucidate the biological mechanism of retinal injury.Methods:Six male C57BL/6J mice,aged 4-6 weeks,were randomly assigned to either the experimental or control group.The experimental group received two injections of magnetic bead suspension into the anterior chamber of a single eye to obstruct the aqueous humor outflow channel,simulating chronic intraocular pressure glaucoma,while the control group received no treatment.RNA-seq and bioinformatics analyses were conducted on the retinas of the experimental and control groups on the 48th day,followed by further analysis of differentially ex-pressed genes(DEGs)and mitochondrial co-expression genes.Results:Following two injections of magnetic beads,mice exhibited increased and sustained intraocular pressure.A total of 606 DEGs were identified in the retinas of mice between the experimental and control groups.GO functional analysis and KEGG pathway analysis of DEGs revealed enrichment in energy metabolism.GSEA analysis showed that oxidative phosphorylation and glycolysis pathways were down-regulated.Fur-ther analysis showed that there were 59 overlapping DEGs and mitochondrial genes,which were en-riched in proton transmembrane transporter activity,mitochondrial protein complex,amino acid degra-dation and so on.PPI network analysis highlighted hub genes as Uqcrfs1,Cyc1,and Atp5f1a.Con-clusion:RNA-seq and bioinformatics analysis suggest energy metabolism disorders play a crucial role in retinal damage and degeneration in chronic hypertensic glaucoma.Furthermore,mitochondrial dys-function,leading to dysregulation in axonal transport,may be a significant factor in the occurrence and development of glaucoma.
TranscriptomeMagnetic Bead ModelGlaucomaMitochondrial DysfunctionAxonal Transport Dysregulation
NETL
NSTL
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