Effect of shikonin on the proliferation and apoptosis of human hypertrophic scar fibroblasts by regulating the Hippo signaling pathway
Objective:To investigate the effect of shikonin on the behavior of human hypertrophic scar fibro-blasts(HSF)and its related molecular mechanisms.Methods:HSF cells were divided into control group,low dose shikonin group,medium dose shikonin group,high dose shikonin group,and XMU-MP-1(Hippo signaling pathway inhibitor)group;CCK-8 was applied to detect cell proliferation;Transwell experiment was applied to detect the migration and invasion abilities of cells in each group;flow cytometry was applied to detect cell apoptosis;Western Blot was applied to detect Col-Ⅰ,Col-Ⅲ,α-SMA,Bcl-2,Bax,YAP,and TAZ proteins.Results:Compared with those in the control group,the cell proliferation rate,numbers of cell migration and invasion,and expression of Col Ⅰ,Col Ⅲ,α-SMA,Bcl-2,YAP,and TAZ proteins in the low,medium,and high dose shikonin groups were significantly decreased,the apoptosis rate and expression of Bax protein were significantly in-creased(P<0.05);there was no statistically significant difference in the above detection indicators of HSF cells between the XMU-MP-1 group and the high-dose shikonin group(P>0.05).Conclusion:Shikonin can inhibit the proliferation and migration of human hypertrophic scar fibroblasts and promote their apoptosis by inhibiting the Hippo signaling pathway.
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维普
