Exploring the relationship between GJB3 and lung cancer metastasis and its clinical significance based on bioinformatics analysis
Objective:To explore new prognostic markers and therapeutic targets associated with epithelial-mesenchymal transition.Methods:Somatic mutation data,methylation data,gene expres-sion profiles,and corresponding clinical information of lung adenocarcinoma(LUAD)patients were obtained from the cancer genome atlas(TCGA)database.We scored the functions related to the epithelial-mesenchymal transition of TCGA patients by the ssGSEA algorithm and analyzed the differences between high-scoring patients and low-scoring patients,therefore screening out the gene GJB3.Next,we examined GJB3's role in the epithelial-mesenchymal transition,lung adenocarcino-ma patient prognosis,DNA methylation,genomic instability,immune infiltration,immunotherapy,and chemotherapy response,and finally verified the association between GJB3 and prognosis of LUAD patients in the GEO database.Results:GJB3 was significantly associated with epithelial-mesenchymal transition.High expression of GJB3 predicted poor overall survival(OS),progress-free interval(PFI),and disease-specific survival(DSS)in LUAD patients.Cox regression analysis showed that GJB3 was an independent biomarker of OS in LUAD patients.In addition,we used three other LUAD datasets to validate the prognostic role of GJB3 in LUAD and obtained simi-lar results.Meta-analysis showed that high GJB3 expression was strongly associated with poor OS.Conclusion:GJB3 is highly expressed in LUAD and promotes its progression.GJB3 may serve as an independent biomarker for predicting the prognosis of lung adenocarcinoma and providing clues for early diagnosis and clinical treatment.
万方数据
维普
