Effects of supramolecular salicylic acid on AMPK/mTOR pathway and autophagy in rats with acne model
Objective:To observe the therapeutic effect of supramolecular salicylic acid(SSA)on rats with acne model induced by Propionibacterium acnes,and to explore its mechanism of action.Methods:Eighteen SD acne model rats were randomly divided into control group,2％SSA group,and 30％SSA group,with 6 rats in each group.The latter two groups were respectively given 2％and 30％SSA for 4 weeks of external use.The skin lesions and histopathological changes in the modeling area were observed in the 2nd and 4th weeks,and the differences in sebum secretion among the three groups were observed by oil red O staining and Nile staining.Western Blot was used to detect AMPK,mTOR,LC3 Ⅱ/Ⅰ,and Beclin1 protein expression in skin lesion tissues in each group.Re-sults:Compared with those in the control group,after 4 weeks,the skin lesions in the 2％SSA group and the 30％SSA group were significantly improved.The sebum secretion of rats was significantly re-duced(P<0.01).The inhibitory effect of lipid secretion in the 30％SSA group was more significant than that in the 2％SSA group(P<0.01).The expression of AMPK,LC3 Ⅱ/Ⅰ,and Beclin1 pro-teins was significantly increased,and the expression of mTOR protein was significantly decreased(P<0.01).Conclusion:Supramolecular salicylic acid can effectively improve acne skin lesions and in-hibit sebaceous gland secretion in ance rats and may play its role in treating acne by promoting the acti-vation of the AMPK-mTOR pathway and promoting cell autophagy.
NETL
NSTL
万方数据
