Method for Extracting Genomic DNA from Leaves of Sweet-potato
The leaf of sweet-potato is rich in polysaccharides, polyphenols and pigments. etc.. The genomic DNA samples of young fresh leaves of sweet-potato were isolated by improved CTAB method. and then the agarose gel electrophoresis and the AFLP analysis were conducted to it. The results showed that each sample of the genomic DNA extracted from 32 leaf samples of sweet-potato by improved CTAB method has some good characters as follows: high purity, good quality, relatively high molecular weight, comparatively clean in removing polysaccharides and polyphenols;the value of OD260/OD230 was higher than 2.0, the value of OD26O/OD28O was between 1.8 to 2.0; non-degradation, and it suitahle for digestion by restriction enzyme. Using primer comhination of E-ACA and M-CTG to do AFLP analysis, and the results showed the genomic DNA has clear bands and good polymorphism. Therefore, the genomic DNA. which is extracted from sweet-potato leaf by this improved CTAB method, is entirely suitahle for the AFLP analysis.
NETL
NSTL
万方数据
维普
