In order to discuss the genetic relationship between bud mutation clones and original variety of New Hall navel orange, young leaves of nine New Hall navel orange varieties and their bud mutation clones were chosen to extract DNA, and then a SRAP-PCR analysis was conducted to them.The results showed that there were differences between each bud mutation clone and control with different extents, but their genetic distance all ranged from 0.100 0 to 0.210 9;the genetic distance between female parent and progeny from the same line was between 0.042 9~0.051 7, and the progenies of each clone were genetically stable;moreover, the genetic distance between female mutation clone with elliptical fruit and mutation clone with round fruit was the largest by 0.257 1.