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植物逆境相关基因密码子的偏好性分析

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运用CodonW1.4.2和SPSS16.0软件对来源于不同植物的17个逆境相关基因的密码子特性进行了分析。结果显示:除CarPRP1基因的ENC值小于40外,其余基因ENC值均介于40~61之间;除CarPRP1基因的GC含量较高外,其余基因的GC含量均在0.35~0.45之间;除CarNAC5基因之外,其他基因密码子第三位碱基的A和T含量远高于C和G含量。这表明:所选逆境相关基因偏爱以A或T结尾的密码子;而且密码子的偏好性与GC含量有关,GC含量越高的基因则密码子使用偏好性越高。聚类分析结果表明:除CarPRP1基因之外,NAC基因家族、CarEP1基因、NHX同源基因、P5CS同源基因、DREB基因相互之间的距离系数相对较小。
Analysis of Codon Bias for Stress Related Genes of Plants
The codon characters of 17 stress related genes from different plants were analyzed by software CodonW1.4.2 and SPSS16.0. The results showed that ENC values of stress related genes were between 40~61, but CarPRP1 gene’s was less than 40; GC contents of stress related genes were between 0.35~0.45, but CarPRP1 gene’s was relatively high; GC3s contents of stress related genes were A/T>C/G except CarNAC5 gene’s. Therefore, the selected stress related genes preferred to use A or T at the end of codon, and the codon bias related to the GC content, in which the higher the GC content in gene was, the higher the codon usage bias was. Moreover, the results of cluster analysis indicated that except CarPRP1 gene, the coefficient of squared Euclidean distance of codon usage bias was relatively small between NAC gene family, CarEP1 gene, NHX genes, P5CS genes and DREB genes.

plantstress related genescodonbiascluster analysis

任燕萍、姚正培、倪志勇、张桦

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新疆农业大学农业生物技术重点实验室,新疆 乌鲁木齐 830052

新疆农业大学农学院,新疆 乌鲁木齐 830052

植物 逆境相关基因 密码子 偏好性 聚类分析

新疆农业大学资助项目

XJAU201101

2014

湖南农业科学
湖南省农业科学院 湖南省科技厅星火促进会 湖南农业大学

湖南农业科学

影响因子:0.415
ISSN:1006-060X
年,卷(期):2014.(9)
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