Analysis of Codon Bias for Stress Related Genes of Plants
The codon characters of 17 stress related genes from different plants were analyzed by software CodonW1.4.2 and SPSS16.0. The results showed that ENC values of stress related genes were between 40~61, but CarPRP1 gene’s was less than 40; GC contents of stress related genes were between 0.35~0.45, but CarPRP1 gene’s was relatively high; GC3s contents of stress related genes were A/T>C/G except CarNAC5 gene’s. Therefore, the selected stress related genes preferred to use A or T at the end of codon, and the codon bias related to the GC content, in which the higher the GC content in gene was, the higher the codon usage bias was. Moreover, the results of cluster analysis indicated that except CarPRP1 gene, the coefficient of squared Euclidean distance of codon usage bias was relatively small between NAC gene family, CarEP1 gene, NHX genes, P5CS genes and DREB genes.
plantstress related genescodonbiascluster analysis