Research Progress of Rice Anther Culture Technology and its Application in Breeding
The paper reviewed the progress in anther culture of rice from genotypes,sampling time,pretreatment,culture mediums,the kinds and ratio of hormones,carbon sources and other additives.Most studies showed anther culture is an effective method to stabilize genetic characters ofIndica/Japonica in rice,but the anther culture plant yield is very low (below 4%) at present,and the rate of Indica is significantly low than that of japonica,the main reason is the low gene affinity and the problem of anther browning and seedling albino.So the paper proposed the improvement of sampling time,pretreatment,culture medium,hormones,additives and other culture conditions,the establishment of anther culture system,combined with transgenic and molecular markers to study the genetic mechanism,and finally to improve the breeding process and efficiency.