Isolation and Identification of an Antagonistic Actinomycete Strain to Phytophthora melonis
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NSTL
维普
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为更好的防治黄瓜疫病，从湖南、贵州等地采集的土壤样品中分离到放线菌106株，采用平板对峙法及发酵液抑菌试验筛选对黄瓜疫霉菌（Phytophthora melonis）具有拮抗活性的放线菌。结果表明，106株放线菌中有26株对黄瓜疫霉菌有抑制作用，抑菌带达到5 mm 及以上的有5株，其中2号菌株的拮抗作用最明显，发酵液稀释5倍后对黄瓜疫霉菌的菌丝生长抑制率仍达76%。对2号菌株进行培养性状、生理生化特征鉴定及16S rDNA 序列分析，初步将其鉴定为绛红褐链霉菌（Streptomyces purpeofuscus）。
In orderto explore biocontrolagentsto controlcucum berphytophthra blightcaused by Phytophthra m elonis,106 actinom ycetes strains w ere isolated from soilsam ples ofH unan and Guizhou provinces.Then dualculture m ethod and ferm entation broth inhibition test w ere used to screen antagonistic actinom ycete strains to Phytophthora m elonis.The results show ed that26 of 106 actinom ycetes strains could inhibitPhytophthora m elonis,5 ofw hich had an inhibition zone over5 m m in w idth.N o.2 strain had the strongestinhibition activity, w hose inhibition rate rem ained 76% even after the ferm entation broth w as diluted 5 tim es. B ased on the identification of cultivation characters as w ellas physiologicaland biochem icalfeatures,and the analysis of16S rD N A sequence,N o.2 strain could be nom enclatured asStreptom ycespurpeofuscus.
Phytophthora m elonis16S rD N Aantagonistic actinom ycetesisolationidentification
NETL
NSTL
维普
万方数据
