黑紫色叶紫薇品种'赤红'叶片转录组测序及注释分析
Sequencing and Annotation of the Leaf Transcriptome of Lagerstroemia indica'Ebony Fire'
刘玉军 1秦波 2李媚 1黄耀恒 2刘芳 1黄欣 2刘莉1
作者信息
- 1. 南宁树木园,广西 南宁 530031
- 2. 广西壮族自治区林业科学研究院,广西特色经济林培育与利用重点实验室,广西 南宁 530002
- 折叠
摘要
对黑紫色叶紫薇品种'赤红'叶片进行高通量测序,并利用Trinity(2.8.6)对测序数据进行从头组装,共获得 23 791条unigene,其中 91.86%的序列在NR、GO、COG、KEGG等 5 大数据库中得到注释;NR数据库比对到的unigene数量最多,占比91.93%,随后依次为SWISS(67.95%)、COG(60.43%)、GO(51.37%)和KEGG(35.43%).在NR数据库中,'赤红'紫薇所有的unigene仅比对到1个物种——石榴(Punica granatum L.),表明同属于千屈菜科的紫薇和石榴亲缘关系较近.从转录组中共筛选到 4 775 个SSR位点,包括 6 种核苷酸重复类型,其中A/T和AG/CT类型占比最高.对色素合成相关通路基因进行分析,共挖掘到18个与类黄酮和花青素相关的基因,其中花青素合成通路中的UGT79B1、BZ1、UGT75C1可能是'赤红'紫薇形成紫黑色叶的关键基因.
Abstract
The high-throughput transcriptome sequencing for the leaves of Lagerstroemia indica'Ebony Fire'yielded 23 791 unigenes after de novo assembly by Trinity(2.8.6).Among them,91.86%of the unigenes were annotated in NR,SWISS,COG,GO,and KEGG.The NR database annotated the largest number of unigenes,which accounted for 91.93%,followed by SWISS(67.95%),COG(60.43%),GO(51.37%),and KEGG(35.43%).Only one species of Punica granatum L.was compared in the NR database,indicating the high sequence homology between L.indica L.and P.granatum L..A total of 4 775 SSR loci were screened out from the transcriptome,involving six nucleotide repeat types,of which A/T and AG/CT accounted for the largest proportion.The mining of genes involved in pigment synthesis revealed 18 genes associated with flavonoids and anthocyanin.UGT79B1,BZ1,and UGT75C1 in the anthocyanin synthesis pathway may be the key genes for L.indica'Ebony Fire'to produce purple black leaves.
关键词
紫薇品种'赤红'/转录组/基因注释/色素合成关键基因Key words
Lagerstroemia indica'Ebony Fire'/transcriptome/gene annotation/key genes in pigment synthesis引用本文复制引用
基金项目
广西自筹经费林业科技项目(桂林科研[2022ZC]第73号)
出版年
2024
国家科技期刊平台
NSTL
万方数据