不同遗传背景的转Cry1C基因玉米品系的室内抗虫性鉴定
Insect Resistance Identification of Transgenic Maize with Cry1C Gene from Different Genetic Backgrounds in Laboratory
曾强 1周伟 2郭欢乐 1曹钟洋 1彭明 1周虹 1汤彬 1陈志辉1
作者信息
- 1. 湖南省作物研究所,湖南 长沙 410125
- 2. 长沙市望城区农业农村局,湖南 长沙 410203
- 折叠
摘要
以转Cry1C基因的转基因玉米C492 为供体,通过回交转育的方式将目标基因分别导入不同遗传背景的玉米自交系,获得转基因玉米自交系C492/N-76、C492/N202、C492/S273、C492/DH1901 和C492/DH1904,采用室内离体组织生测的方法研究各品系对玉米螟和草地贪夜蛾的抗虫性.结果表明:转基因玉米C492和5个不同遗传背景自交系均表现出高抗玉米螟,虽对草地贪夜蛾的抗性有一定差异,但接虫 6d后幼虫死亡率均达到 80%以上.这说明转基因玉米C492 和 5 个不同遗传背景自交系可用于抗虫转基因玉米新品种培育,具有较好的商业化应用前景.
Abstract
The transgenic maize C492 carrying the Cry1C gene was used as the donor,and the target gene was introduced into inbred lines of maize with different genetic backgrounds by backcross breeding.The inbred lines of transgenic maize,namely C492/N-76,C492/N202,C492/S273,C492/DH1901,and C492/DH1904 were obtained.The insect resistance of the inbred lines to Ostrinia furnacalis and Spodoptera frugiperda was studied through in vitro tissue bioassay in the laboratory.The results show that transgenic maize C492 and the five inbred lines with different genetic backgrounds are highly resistant to O.furnacalis.Although there is a difference in their resistance to S.frugiperda,a larval mortality rate of over 80%can be achieved after insect infestation for six days.This indicates that transgenic maize C492 and the five inbred lines with different genetic backgrounds can be used for the breeding of new insect-resistant transgenic maize varieties and have good prospects for commercial application.
关键词
转基因玉米/亚洲玉米螟/草地贪夜蛾/回交转育/室内生测Key words
transgenic maize/Ostrinia furnacalis/Spodoptera frugiperda/backcross breeding/bioassay in laboratory引用本文复制引用
基金项目
湖南省农业科技创新资金项目(2022CX108)
湖南省农业种质资源保护与利用项目(湘财农指[2023]0043号)
湖南省现代农业产业技术体系建设项目(湘农发[2019]105号)
国家玉米产业技术体系建设项目长沙综合试验站项目(CARS-02)
出版年
2024
国家科技期刊平台
NSTL
万方数据